Chapter title |
Immunoglobulin/T-Cell Receptor Gene Rearrangement Analysis Using RNA-Seq
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Book title |
Immunogenetics
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Published in |
Methods in molecular biology, January 2022
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DOI | 10.1007/978-1-0716-2115-8_4 |
Pubmed ID | |
Book ISBNs |
978-1-07-162114-1, 978-1-07-162115-8
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Authors |
van der Velden, Vincent H J, Bastian, Lorenz, Brüggemann, Monika, Hartmann, Alina M, Darzentas, Nikos, Vincent H. J. van der Velden, Lorenz Bastian, Monika Brüggemann, Alina M. Hartmann, Nikos Darzentas |
Abstract |
Identification of immunoglobulin (IG) and T-cell receptor (TR) gene rearrangements in acute lymphoblastic leukemia (ALL) patients at initial presentation are crucial for monitoring of minimal residual disease (MRD) during subsequent follow-up and thereby for appropriate risk-group stratification. Here we describe how RNA-Seq data can be generated and subsequently analyzed with ARResT/Interrogate to identify possible MRD markers. In addition to the procedures, possible pitfalls will be discussed. Similar strategies can be employed for other lymphoid malignancies, such as lymphoma and myeloma. |
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