Plant Gene Silencing
Springer US
Chapter title |
Trans-Kingdom RNA Silencing in Plant-Fungal Disease Control.
|
---|---|
Book title |
Plant Gene Silencing
|
Published in |
Methods in molecular biology, January 2022
|
DOI | 10.1007/978-1-0716-1875-2_16 |
Pubmed ID | |
Book ISBNs |
978-1-07-161874-5, 978-1-07-161875-2
|
Authors |
Zhang, Tao, Wang, Fei, Guo, Hui-Shan, Jin, Yun |
Abstract |
Trans-kingdom RNA interference (RNAi) has been reported in several plant-fungal pathosystems. Our recent works have demonstrated natural RNAi transmission from cotton plants into Verticillium dahliae, a soil-borne phytopathogenic fungus that infects host roots and proliferates in vascular tissues, and successful application of trans-kingdom RNAi in cotton plants to confer Verticillium wilt disease resistance. Here, we provide a detailed protocol of cotton infection with V. dahliae, fungal hyphae recovery from infected cotton stems, and transmitted small RNA detection developed from our previous studies for trans-kingdom RNAi assays. |
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Type | Count | As % |
---|---|---|
Science communicators (journalists, bloggers, editors) | 1 | 50% |
Members of the public | 1 | 50% |
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 1 | 100% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 1 | 100% |