Brown Adipose Tissue
Springer US
Chapter title |
Analysis of Single-Cell/Nucleus Transcriptome Data in Adipose Tissue.
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Book title |
Brown Adipose Tissue
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Published in |
Methods in molecular biology, January 2022
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DOI | 10.1007/978-1-0716-2087-8_19 |
Pubmed ID | |
Book ISBNs |
978-1-07-162086-1, 978-1-07-162087-8
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Authors |
Sun, Wenfei, Wenfei Sun |
Abstract |
Adipose tissue is highly heterogeneous and plastic. Recent advances in single-cell/nucleus RNA sequencing technology have helped to study the cellular composition and dynamics of adipose tissue. In this protocol, I outline a typical workflow of analyzing single-cell/nucleus transcriptome data. Specifically, I show an example of how cellular populations are estimated and characterized from a single-nucleus RNAseq data set of frozen archived human adipose tissue. |
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United States | 1 | 25% |
Unknown | 3 | 75% |
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Scientists | 2 | 50% |
Members of the public | 2 | 50% |