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Antigen Processing

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Cover of 'Antigen Processing'

Table of Contents

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    Book Overview
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    Chapter 1 Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes
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    Chapter 2 Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry
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    Chapter 3 Antigen Processing
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    Chapter 4 Kinetic Studies of Cytoplasmic Antigen Processing and Production of MHC Class I Ligands
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    Chapter 5 Assaying Peptide Translocation by the Peptide Transporter TAP
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    Chapter 6 In Vitro Reconstitution of the MHC Class I Peptide-Loading Complex
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    Chapter 7 Studying MHC Class I Peptide Loading and Exchange In vitro
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    Chapter 8 Measuring Synthesis and Degradation of MHC Class I Molecules
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    Chapter 9 Studying ubiquitination of MHC class I molecules.
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    Chapter 10 Evaluation of Viral Interference with MHC Class I-Restricted Antigen Processing and Presentation Using a Flow Cytometry-Based Approach
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    Chapter 11 Determining the Activity of the Transporter Associated with Antigen Processing in the Compartments of the Secretory Pathway
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    Chapter 12 Biochemical Large-Scale Identification of MHC Class I Ligands
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    Chapter 13 Establishing MHC Class I Peptide Motifs
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    Chapter 14 Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies
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    Chapter 15 Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules
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    Chapter 16 Identifying source proteins for MHC class I-presented peptides.
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    Chapter 17 Purification, preparation, and use of chaperone-Peptide complexes for tumor immunotherapy.
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    Chapter 18 Recombinant Poxviruses: Versatile Tools for Immunological Assays
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    Chapter 19 Bioinformatics Identification of Antigenic Peptide: Predicting the Specificity of Major MHC Class I and II Pathway Players
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    Chapter 20 Evaluating CD8 + T Cell Responses In Vitro
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    Chapter 21 Cloning CD8 + Cytolytic T Lymphocytes
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    Chapter 22 Production of CD4 + and CD8 + T Cell Hybridomas
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    Chapter 23 Tracking Antigen-Specific CD8 + T Cells Using MHC Class I Multimers
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    Chapter 24 The Purification of Large Numbers of Antigen Presenting Dendritic Cells from Mouse Spleen
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    Chapter 25 Preparation of Dendritic Cells by In Vitro Cultures
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    Chapter 26 Monitoring Dendritic Cell Activation and Maturation
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    Chapter 27 Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy
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    Chapter 28 Isolation of a Specialized, Antigen-Loaded Early Endosomal Subpopulation by Flow Cytometry
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    Chapter 29 Preparing Antigens Suitable for Cross-presentation Assays In Vitro and In Vivo
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    Chapter 30 Gene Transduction in Human Monocyte-Derived Dendritic Cells Using Lentiviral Vectors
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    Chapter 31 Pulse-Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading.
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    Chapter 32 Assembly of Matched Alpha/Beta Subunits to HLA Class II Peptide Receptors
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    Chapter 33 Studying MHC Class II Peptide Loading and Editing In Vitro
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    Chapter 34 Study of the Allogeneic Response Induced by Endothelial Cells Expressing HLA Class II After Lentiviral Transduction
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    Chapter 35 Antigen Processing for MHC Presentation via Macroautophagy.
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    Chapter 36 Studying MHC Class II Transport in Dendritic Cells
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    Chapter 37 In Vitro Digestion with Proteases Producing MHC Class II Ligands
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    Chapter 38 MHC-II Ubiquitination
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    Chapter 39 Studying MHC class II Presentation of Immobilized Antigen by B Lymphocytes
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    Chapter 40 Production of Primary Human CD4 + T Cell Lines and Clones
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    Chapter 41 Analyzing Antigen Recognition by Natural Killer T Cells
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    Chapter 42 Chromatofocusing Purification of CD1b–Antigen Complexes and Their Analysis by Isoelectric Focusing
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    Chapter 43 Erratum: Production of CD4 + and CD8 + T Cell Hybridomas
Attention for Chapter 16: Identifying source proteins for MHC class I-presented peptides.
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Chapter title
Identifying source proteins for MHC class I-presented peptides.
Chapter number 16
Book title
Antigen Processing
Published in
Methods in molecular biology, January 2013
DOI 10.1007/978-1-62703-218-6_16
Pubmed ID
Book ISBNs
978-1-62703-217-9, 978-1-62703-218-6
Authors

Nathalie Vigneron, Wenbin Ma, Alexandre Michaux, Benoît J. Van den Eynde, Vigneron, Nathalie, Ma, Wenbin, Michaux, Alexandre, Eynde, Benoît J. Van den

Abstract

Identification of antigenic peptides recognized by cytolytic T lymphocytes (CTL) is a prerequisite for the development of targeted cancer immunotherapy approaches. This chapter provides a global approach for the identification of peptides recognized by CTL. It implies the identification of the HLA molecule presenting the peptide as well as the design and screening of a cDNA library derived from the tumor cells.

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The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 33%
Other 1 33%
Unknown 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Immunology and Microbiology 1 33%
Unknown 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2013.
All research outputs
#18,326,065
of 22,693,205 outputs
Outputs from Methods in molecular biology
#7,835
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Outputs of similar age
#217,965
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Outputs of similar age from Methods in molecular biology
#219
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