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High-Throughput RNAi Screening

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Cover of 'High-Throughput RNAi Screening'

Table of Contents

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    Book Overview
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    Chapter 1 High-Throughput RNAi Screening
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    Chapter 2 Optimization of Transfection Conditions for siRNA Screening.
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    Chapter 3 High-Throughput RNAi Screening
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    Chapter 4 High-Throughput RNAi Screening
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    Chapter 5 Pooled shRNA Screening in Mammalian Cells as a Functional Genomic Discovery Platform.
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    Chapter 6 A Protocol for a High-Throughput Multiplex Cell Viability Assay.
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    Chapter 7 RNAi Screening of Leukemia Cells Using Electroporation.
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    Chapter 8 Self-Assembled Cell Microarray (SAMcell) for High-Throughput RNAi Screening.
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    Chapter 9 High-Throughput RNAi Screening
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    Chapter 10 High-Throughput RNAi Screening
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    Chapter 11 High-Throughput RNAi Screening
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    Chapter 12 High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans.
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    Chapter 13 High-Throughput RNAi Screening
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    Chapter 14 High-Throughput RNAi Screening
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    Chapter 15 High-Throughput RNAi Screening
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    Chapter 16 High-Throughput RNAi Screening
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    Chapter 17 Morphology and Gene Expression Screening with Morpholinos in Zebrafish Embryos
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    Chapter 18 Live Cell Microscopy-Based RNAi Screening in the Moss Physcomitrella patens.
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    Chapter 19 Data Analysis for High-Throughput RNAi Screening.
Attention for Chapter 12: High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans.
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Chapter title
High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans.
Chapter number 12
Book title
High-Throughput RNAi Screening
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6337-9_12
Pubmed ID
27581291
Book ISBNs
978-1-4939-6335-5, 978-1-4939-6337-9
Authors

Linda P. O’Reilly, Ryan R. Knoerdel, Gary A. Silverman, Stephen C. Pak

Editors

David O. Azorsa, Shilpi Arora

Abstract

RNA interference (RNAi) is a process in which double-stranded RNA (dsRNA) molecules mediate the inhibition of gene expression. RNAi in C. elegans can be achieved by simply feeding animals with bacteria expressing dsRNA against the gene of interest. This "feeding" method has made it possible to conduct genome-wide RNAi experiments for the systematic knockdown and subsequent investigation of almost every single gene in the genome. Historically, these genome-scale RNAi screens have been labor and time intensive. However, recent advances in automated, high-throughput methodologies have allowed the development of more rapid and efficient screening protocols. In this report, we describe a fast and efficient, liquid-based method for genome-wide RNAi screening.

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The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 19%
Student > Ph. D. Student 2 13%
Student > Postgraduate 2 13%
Student > Master 2 13%
Other 1 6%
Other 3 19%
Unknown 3 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 25%
Medicine and Dentistry 3 19%
Agricultural and Biological Sciences 2 13%
Neuroscience 2 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Other 1 6%
Unknown 3 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 December 2017.
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#18,469,995
of 22,886,568 outputs
Outputs from Methods in molecular biology
#7,923
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Outputs of similar age from Methods in molecular biology
#845
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