Chapter title |
Construction of Genomic and Sub-Genomic Dengue Infectious Replicons.
|
---|---|
Book title |
Dengue Virus
|
Published in |
Methods in molecular biology, January 2022
|
DOI | 10.1007/978-1-0716-1879-0_7 |
Pubmed ID | |
Book ISBNs |
978-1-07-161878-3, 978-1-07-161879-0
|
Authors |
Cao, Thu M, Kuhn, Richard J, Cao, Thu M., Kuhn, Richard J. |
Abstract |
Dengue replicons are powerful tools used in studying virus biology as well as in high-throughput screening of drug candidates. Replicon constructs are developed as genomic (consists of all the viral protein genes) or sub-genomic (consists of only nonstructural protein genes) and are used to study various aspects of the virus life cycle such as genome replication and virus assembly. In addition, a replicon usually includes a reporter gene used in monitoring virus replication. In this chapter, we provide methods to develop both genomic and sub-genomic dengue replicons with a luciferase reporter and describe different assays to utilize these systems. |
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Mendeley readers
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Student > Ph. D. Student | 1 | 20% |
Researcher | 1 | 20% |
Student > Doctoral Student | 1 | 20% |
Student > Master | 1 | 20% |
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Agricultural and Biological Sciences | 1 | 20% |
Unknown | 1 | 20% |