Chapter title |
In-cell NMR in Mammalian cells: part 3.
|
---|---|
Chapter number | 6 |
Book title |
Intrinsically Disordered Protein Analysis
|
Published in |
Methods in molecular biology, July 2012
|
DOI | 10.1007/978-1-61779-927-3_6 |
Pubmed ID | |
Book ISBNs |
978-1-61779-926-6, 978-1-61779-927-3
|
Authors |
Bekei B, Rose HM, Herzig M, Stephanowitz H, Krause E, Selenko P, Beata Bekei, Honor May Rose, Michaela Herzig, Heike Stephanowitz, Eberhard Krause, Philipp Selenko |
Abstract |
Irrespective of how isotope-labeled proteins are delivered into mammalian cells, laboratory routines are needed to assess the quality of the resulting in-cell NMR samples. These include methods to evaluate overall cell viability, protein transduction efficiency, intracellular protein concentration, localization, and stability. In addition, quality control experiments to assess protein leakage from manipulated cells are of particular importance for in-cell NMR experiments. The purpose of this chapter is to outline qualitative and quantitative methods to determine general biological properties of in-cell NMR samples in order to ensure the highest possible standards for in-cell NMR studies. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 5% |
Unknown | 20 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 7 | 33% |
Student > Ph. D. Student | 5 | 24% |
Other | 2 | 10% |
Professor | 2 | 10% |
Student > Postgraduate | 2 | 10% |
Other | 2 | 10% |
Unknown | 1 | 5% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 8 | 38% |
Agricultural and Biological Sciences | 6 | 29% |
Chemistry | 6 | 29% |
Unknown | 1 | 5% |