| Chapter title |
High-Throughput RNAi Screening
|
|---|---|
| Chapter number | 10 |
| Book title |
High-Throughput RNAi Screening
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-6337-9_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6335-5, 978-1-4939-6337-9
|
| Authors |
Iles, LaKesla R, Bartholomeusz, Geoffrey A, LaKesla R. Iles, Geoffrey A. Bartholomeusz Ph.D., Iles, LaKesla R., Bartholomeusz, Geoffrey A., Geoffrey A. Bartholomeusz |
| Editors |
David O. Azorsa, Shilpi Arora |
| Abstract |
The intrinsic limitations of 2D monolayer cell culture models have prompted the development of 3D cell culture model systems for in vitro studies. Multicellular tumor spheroid (MCTS) models closely simulate the pathophysiological milieu of solid tumors and are providing new insights into tumor biology as well as differentiation, tissue organization, and homeostasis. They are straightforward to apply in high-throughput screens and there is a great need for the development of reliable and robust 3D spheroid-based assays for high-throughput RNAi screening for target identification and cell signaling studies highlighting their potential in cancer research and treatment. In this chapter we describe a stringent standard operating procedure for the use of MCTS for high-throughput RNAi screens. |
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|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 50% |
| Student > Master | 3 | 30% |
| Student > Postgraduate | 1 | 10% |
| Unknown | 1 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Chemical Engineering | 1 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 10% |
| Agricultural and Biological Sciences | 1 | 10% |
| Medicine and Dentistry | 1 | 10% |
| Other | 2 | 20% |
| Unknown | 2 | 20% |