Chapter title |
Oxygen Transport to Tissue XXXVIII
|
---|---|
Chapter number | 38 |
Book title |
Oxygen Transport to Tissue XXXVIII
|
Published in |
Advances in experimental medicine and biology, January 2016
|
DOI | 10.1007/978-3-319-38810-6_38 |
Pubmed ID | |
Book ISBNs |
978-3-31-938808-3, 978-3-31-938810-6
|
Authors |
Li, Fang-Hui, Yu, Hai-Tao, Xiao, Lin, Liu, Yan-Ying, Fang-Hui Li, Hai-Tao Yu, Lin Xiao, Yan-Ying Liu |
Editors |
Qingming Luo, Lin Z. Li, David K. Harrison, Hua Shi, Duane F. Bruley |
Abstract |
The aim of this study was to analyze the effects of exercise training on Bax and Bcl-2 protein content and sirtuin1 (SIRT1) mRNA expression levels to prevent sarcopenia in aging rats. Eight 18 months old male Sprague-Dawley rats were trained 5 days weekly for 8 weeks on a treadmill, and eight sedentary rats served as controls. Gastrocnemius muscles were dissected 2 days after the last training session. The mRNA content of PGC-1α, caspase-3, NRF1, TFAM, SOD2, and SIRT1 was estimated by RT-PCR with GAPDH used as an internal control. The protein expression of BAX and Bcl-2 was assessed by Western immunoblot. After training, significant (p < 0.05) increases were noted for the gastrocnemius muscle weights, the gastrocnemius mass/body mass ratio, the bcl-2/BAX ratio, the Bcl-2 protein and the SIRT1, PGC-1α, NRF1, TFAM, SOD2 mRNA content in the trained gastrocnemius, relative to the control samples. No difference was found in the BAX protein between control and trained muscles, whereas the caspase-3 mRNA content decreased by 50 %, in the gastrocnemius muscle of trained animals. Exercise training may inhibit age-induced myonuclear apoptosis by stimulating SIRT1/PGC-1α mRNA expression, thereby preventing sarcopenia in aging rat. |
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