Chapter title |
Cilia
|
---|---|
Chapter number | 3 |
Book title |
Cilia
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3789-9_3 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3787-5, 978-1-4939-3789-9
|
Authors |
Ghossoub, Rania, Lindbæk, Louise, Molla-Herman, Anahi, Schmitt, Alain, Christensen, Søren Tvorup, Benmerah, Alexandre, Rania Ghossoub, Louise Lindbæk, Anahi Molla-Herman, Alain Schmitt, Søren Tvorup Christensen, Alexandre Benmerah |
Abstract |
In many vertebrate cell types, the proximal part of the primary cilium is positioned within an invagination of the plasma membrane known as the ciliary pocket. Recent evidence points to the conclusion that the ciliary pocket comprises a unique site for exocytosis and endocytosis of ciliary proteins, which regulates the spatiotemporal trafficking of receptors into and out of the cilium to control its sensory function. In this chapter, we provide methods based on electron microscopy, 3D reconstruction of fluorescence images as well as live cell imaging suitable for investigating processes associated with endocytosis at the ciliary pocket. |
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