| Chapter title |
Plant Cytogenetics
|
|---|---|
| Chapter number | 9 |
| Book title |
Plant Cytogenetics
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3622-9_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3620-5, 978-1-4939-3622-9
|
| Authors |
Luo, Ming-Cheng, Deal, Karin R, Murray, Armond, Zhu, Tingting, Hastie, Alex R, Stedman, Will, Sadowski, Henry, Saghbini, Michael, Ming-Cheng Luo, Karin R. Deal, Armond Murray, Tingting Zhu, Alex R. Hastie, Will Stedman, Henry Sadowski, Michael Saghbini, Deal, Karin R., Hastie, Alex R. |
| Abstract |
Application of optical mapping based on BioNano Genomics Irys(®) technology ( http://www.bionanogenomics.com/ ) is growing rapidly since its debut in November 2012. The technology can be used to facilitate genome sequence assembly and analysis of genome structural variations. We describe here the detailed protocol that we used to generate a whole genome BioNano map for Aegilops tauschii, the D genome progenitor of hexaploid wheat (Triticum aestivum). We are using the whole genome BioNano map to validate sequence assembly based on the next-generation sequencing, order sequence scaffolds, and ultimately build pseudomolecules for the genome. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 43% |
| Student > Master | 2 | 14% |
| Student > Doctoral Student | 1 | 7% |
| Researcher | 1 | 7% |
| Unknown | 4 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 57% |
| Biochemistry, Genetics and Molecular Biology | 2 | 14% |
| Unknown | 4 | 29% |