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MicroRNA Protocols

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Cover of 'MicroRNA Protocols'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 The MicroRNA
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    Chapter 2 Analysis of MicroRNA Length Variety Generated by Recombinant Human Dicer
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    Chapter 3 A User-Friendly Computational Workflow for the Analysis of MicroRNA Deep Sequencing Data
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    Chapter 4 The RNA Gene Information: Retroelement-MicroRNA Entangling as the RNA Quantum Code
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    Chapter 5 Setting Up an Intronic miRNA Database
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    Chapter 6 Identify Intronic MicroRNA with Bioinformatics
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    Chapter 7 Experimental miRNA Target Validation
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    Chapter 8 Enrichment Analysis of miRNA Targets
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    Chapter 9 MicroRNA Expression Profiling During Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells.
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    Chapter 10 Evaluating the MicroRNA Targeting Sites by Luciferase Reporter Gene Assay.
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    Chapter 11 Expression Pattern Analysis of MicroRNAs in Caenorhabditis elegans
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    Chapter 12 Use of Viral Systems to Study miRNA-Mediated Regulation of Gene Expression in Human Cells.
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    Chapter 13 Cloning, Expression, and Functional Analysis of Genomic miRNA Using Retroviral System in Cancer Cells
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    Chapter 14 Preparing Synaptoneurosomes from Adult Mouse Forebrain
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    Chapter 15 Isolation of Total RNA and Detection Procedures for miRNA Present in Bovine-Cultured Adipocytes and Adipose Tissues
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    Chapter 16 miRNA and shRNA Expression Vectors Based on mRNA and miRNA Processing.
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    Chapter 17 Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs.
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    Chapter 18 Vector-Free Methods for Manipulating miRNA Activity In Vitro and In Vivo
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    Chapter 19 MicroRNA Expression Profiling of Human-Induced Pluripotent and Embryonic Stem Cells
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    Chapter 20 MicroRNA Expression During Neuronal Differentiation of Human Teratocarcinoma NTera2D1 and Mouse Embryonic Carcinoma P19 Cells
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    Chapter 21 Isolation and Identification of Gene-Specific MicroRNAs
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    Chapter 22 Transgene-Like Animal Models Using Intronic MicroRNAs.
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    Chapter 23 Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR-302 Induction.
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    Chapter 24 Salivary MicroRNAs and Oral Cancer Detection
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    Chapter 25 Application of Intronic MicroRNA Agents in Cosmetics
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    Chapter 26 MicroRNAs in Skin and Wound Healing
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    Chapter 27 MiRNA Targets of Prostate Cancer.
Attention for Chapter 9: MicroRNA Expression Profiling During Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells.
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Chapter title
MicroRNA Expression Profiling During Neural Differentiation of Mouse Embryonic Carcinoma P19 Cells.
Chapter number 9
Book title
MicroRNA Protocols
Published in
Methods in molecular biology, September 2012
DOI 10.1007/978-1-62703-083-0_9
Pubmed ID
Book ISBNs
978-1-62703-082-3, 978-1-62703-083-0
Authors

Botao Zhao, Bing Huang, Wei Li, Youxin Jin, Zhao, Botao, Huang, Bing, Li, Wei, Jin, Youxin

Abstract

The Mouse P19 cell line was derived from an embryonal carcinoma. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells. Here, we describe an miRNA microarray method to monitor the miRNA expression profiles during the course of P19 cells neuronal differentiation. The results indicated that there were significant differences between the miRNA expression in P19 EC cells and the resultant differentiated neural stem cells.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
China 2 4%
Portugal 1 2%
Unknown 43 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 18 39%
Student > Ph. D. Student 15 33%
Professor > Associate Professor 4 9%
Student > Postgraduate 3 7%
Professor 2 4%
Other 3 7%
Unknown 1 2%
Readers by discipline Count As %
Agricultural and Biological Sciences 28 61%
Biochemistry, Genetics and Molecular Biology 8 17%
Medicine and Dentistry 4 9%
Neuroscience 2 4%
Social Sciences 1 2%
Other 1 2%
Unknown 2 4%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 November 2013.
All research outputs
#18,319,742
of 22,684,168 outputs
Outputs from Methods in molecular biology
#7,832
of 13,045 outputs
Outputs of similar age
#130,416
of 171,824 outputs
Outputs of similar age from Methods in molecular biology
#42
of 77 outputs
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