Chapter title |
Cartilage Tissue Engineering
|
---|---|
Chapter number | 2 |
Book title |
Cartilage Tissue Engineering
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2938-2_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2937-5, 978-1-4939-2938-2
|
Authors |
Shahin, Kifah, Mahmoudifar, Nastaran, Doran, Pauline M, Doran, Pauline M., Kifah Shahin, Nastaran Mahmoudifar, Pauline M. Doran |
Abstract |
As the only cell type found in healthy adult cartilage, chondrocytes are the obvious and most direct starting point for cartilage tissue engineering. Human adult, juvenile, neonatal, and fetal chondrocytes have all been demonstrated to produce cartilage matrix components in vitro for production of engineered tissues. In this chapter, procedures are outlined for isolation of chondrocytes from human fetal and adult cartilage. Methods for expansion and cryopreservation of the cells and characterization of gene expression using quantitative polymerase chain reaction (Q-PCR) analysis are also described. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Other | 1 | 13% |
Unknown | 7 | 88% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 1 | 13% |
Unknown | 7 | 88% |