| Chapter title |
Skeletal Muscle Regeneration in the Mouse
|
|---|---|
| Chapter number | 11 |
| Book title |
Skeletal Muscle Regeneration in the Mouse
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3810-0_11 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3808-7, 978-1-4939-3810-0
|
| Authors |
Vogler, Thomas O, Gadek, Katherine E, Cadwallader, Adam B, Elston, Tiffany L, Olwin, Bradley B, Thomas O. Vogler, Katherine E. Gadek, Adam B. Cadwallader, Tiffany L. Elston, Bradley B. Olwin, Vogler, Thomas O., Gadek, Katherine E., Cadwallader, Adam B., Elston, Tiffany L., Olwin, Bradley B. |
| Editors |
Michael Kyba |
| Abstract |
Adult skeletal muscle stem cells, termed satellite cells, regenerate and repair the functional contractile cells in adult skeletal muscle called myofibers. Satellite cells reside in a niche between the basal lamina and sarcolemma of myofibers. Isolating single myofibers and their associated satellite cells provides a culture system that partially mimics the in vivo environment. We describe methods for isolating and culturing intact individual myofibers and their associated satellite cells from the mouse extensor digitorum longus muscle. Following dissection and isolation of individual myofibers we provide protocols for myofiber transplantation, satellite cell transfection, immune detection of satellite cell antigens, and assays to examine satellite cell self-renewal and proliferation. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 33% |
| United Kingdom | 1 | 17% |
| Taiwan | 1 | 17% |
| Japan | 1 | 17% |
| Unknown | 1 | 17% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 67% |
| Scientists | 1 | 17% |
| Practitioners (doctors, other healthcare professionals) | 1 | 17% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 27% |
| Researcher | 4 | 13% |
| Student > Master | 3 | 10% |
| Student > Doctoral Student | 2 | 7% |
| Student > Bachelor | 2 | 7% |
| Other | 5 | 17% |
| Unknown | 6 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 37% |
| Agricultural and Biological Sciences | 5 | 17% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 10% |
| Environmental Science | 1 | 3% |
| Medicine and Dentistry | 1 | 3% |
| Other | 2 | 7% |
| Unknown | 7 | 23% |